禽呼肠孤病毒感染对SPF鸡外周血淋巴细胞中天然免疫相关基因转录的影响

doi:10.11843/j.issn.0366-6964.2017.01.014

Abstract

Abstract:

This study was aimed to assess the influences of avian reovirus (ARV) infection on expression of innate immune genes in peripheral blood lymphocytes of SPF chickens.The quantitative real-time PCR assays was performed to determine the transcriptional levels of TLR3, TLR7, TLR21, MDA5, IPS-1, IRF-3, IFN-α, IFN-β, IFN-γ, IFITM3, Mx1 and OASL in 0 h, 12 h, 1, 2, 3, 5 and 7 days post infection (dpi) in peripheral blood lymphocytes of SPF chickens infected with ARV S1133. These results showed that the mRNA transcriptional levels of TLR3 and TLR21 were significantly reduced within 7 dpi （P<0.05 or P<0.01）; the mRNA transcriptional levels of TLR7 and MDA5 were obviously promoted and specially reached the summit maximum values on 7 dpi and 1 dpi, respectively (P<0.05 or P<0.01). The mRNA level of IPS-1 was down-regulated within 7 dpi. Meanwhile, the mRNA level of IRF-3 was up-regulated during the whole transcrption course, and IFN-α, IFN-β and IFN-γ mRNA levels were significantly increased and reached the peak at 2-3 dpi. The mRNA levels of IFITM3, Mx1 and OASL were significantly up-regulated and peaked at 3 dpi (P<0.01). Therefore, ARV infection is able to modulate the increased mRNA transcription of TLR7, MDA5, IRF-3, IFN-α, IFN-β, IFN-γ, IFITM3, Mx1 and OASL, and reduce the mRNA transcription of TLR3, TLR21 and IPS-1, which would contribute to further understand ARV pathogenesis and host innate immune responses to ARV infection.

CLC Number:

S852.659.4

HUANG Li,XIE Zhi-xun, LAN Yuan-xi,XIE Li-ji, DENG Xian-wen, FAN Qing, LUO Si-si, XIE Zhi-qin, HUANG Jiao-ling, ZHANG Yan-fang, WANG Sheng, ZENG Ting-ting. Effects of Avian Reovirus Infection on Transcription of Innate Immune Genes in Peripheral Blood Lymphocytes of SPF Chickens[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(1): 116-123.

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Effects of Avian Reovirus Infection on Transcription of Innate Immune Genes in Peripheral Blood Lymphocytes of SPF Chickens

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